| 質粒名稱: | Beta-arrestin2 GFP WT |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 35411 |
| 存儲實驗室: | Robert Lefkowitz |
|
載體骨架基本信息 |
|
| 載體名稱: | pEGFP-N1 |
| 載體出品公司: | Clontech |
| 載體抗性: | 卡那霉素 |
| 空載體大小: | 4700 bp |
| 完整質粒大小: | -- |
| 載體修飾: | -- |
| 載體類型: | 哺乳細胞表達 |
| 篩選標記: | -- |
| 高拷貝/低拷貝: | 高拷貝 |
| 生長條件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名稱: | βarrestin2 |
| 物種來源: | R. norvegicus (rat) |
| 插入大小: | 1500 bp |
| 基因突變: | -- |
| GenBank編號 | -- |
| 啟動子 | CMV |
| 標簽/融合蛋白: | EGFP（主干上的C端） |
| 克隆方法: | 限制性內切酶 |
| 5' 克隆位點: | HindIII(未破壞) |
| 3' 克隆位點: | ApaI(未破壞) |
| 5' 測序引物 | -- |
| 3' 測序引物 | EGFP-N |
|
文獻引用方法 |
|
| 材料和方法部分: | Beta-arrestin2 GFP WT was a gift from Robert Lefkowitz (Addgene plasmid # 35411 ; //n2t.net/addgene:35411 ; RRID:Addgene_35411) |
| 參考文獻部分: | Receptor-specific ubiquitination of beta-arrestin directs assembly and targeting of seven-transmembrane receptor signalosomes. Shenoy SK, Lefkowitz RJ. J Biol Chem. 2005 Apr 15;280(15):15315-24. Epub 2005 Feb 7. 10.1074/jbc.M412418200 PubMed 15699045 |
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Beta-arrestin2 GFP WT載體質粒基本信息
Beta-arrestin2 GFP WT質粒圖譜載體圖譜和Beta-arrestin2 GFP WT載體序列質粒序列多克隆位點信息
Beta-arrestin2 GFP WT質粒載體簡介
Beta-arrestin2 GFP WT質粒序列載體序列
LOCUS Beta-arrestin2 GFP WT 5954 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Beta-arrestin2 GFP WT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5954)
AUTHORS Shenoy SK, Lefkowitz RJ
TITLE Receptor-specific ubiquitination of beta-arrestin directs assembly
and targeting of seven-transmembrane receptor signalosomes.
JOURNAL J Biol Chem. 2005 Apr 15;280(15):15315-24. Epub 2005 Feb 7.
PUBMED 15699045
REFERENCE 2 (bases 1 to 5954)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
//www.snapgene.com
FEATURES Location/Qualifiers
source 1..5954
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..304
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 305..508
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 459..479
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 1834..1843
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1840..2559
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(1885..1906)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2146..2165)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 2493..2514
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 2682..2803
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2719..2738)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2773..2792
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2810..3265)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2947..2968)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3159..3178
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3292..3396
/gene="bla"
/label=AmpR promoter
promoter 3398..3755
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(3421..3441)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3606..3741
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3668..3687
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3790..4584
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3844..3863)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4454..4473
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4772..4791)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4816..4863
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5192..5780
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5681..5700
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
541 ccggactcag atctcgagct caagcttggt accacgcgca ccatgggtga aaaacccggg
601 accagggtct tcaagaagtc gagccctaac tgcaagctca ccgtgtactt gggcaagcgt
661 gactttgtgg atcacttgga caaagtggat cctgtcgatg gtgtggtgct tgtggatcct
721 gactacttga aggaccggaa agtgtttgtg accctcacct gtgccttccg ctatggccga
781 gaagacctgg atgtactggg cctgtctttc cgcaaagatc tgttcatcgc cacctaccag
841 gccttccccc ccatgcccaa cccacctcgg ccccccaccc gcctacagga ccgactgctg
901 aagaagttgg gccagcacgc ccaccccttt tttttcacaa taccccagaa tttgccttgc
961 tccgtcacac tgcagccagg accggaggac acagggaagg cctgtggagt agactttgag
1021 attcgagcct tctgtgccaa atctatagaa gaaaaaagcc acaaaaggaa ctccgtgcgg
1081 cttatcatca gaaaggtaca gtttgctcct gagacacccg gcccccagcc atcagctgaa
1141 accacacgcc acttcctcat gtctgaccgg aggtccctgc acctagaggc ttccctggac
1201 aaagagctgt actaccatgg ggaacccctc aatgtcaacg tccacgtcac caacaattct
1261 gccaaaaccg tcaagaagat cagagtgtct gtgagacagt atgccgacat ttgcctcttc
1321 agcaccgcgc agtacaagtg tcctgtggct cagcttgaac aagatgacca ggtgtctccc
1381 agttccacat tctgcaaggt gtacaccata accccgctgc tcagtgacaa ccgagagaag
1441 cgtggccttg cccttgatgg gcaactcaag cacgaagaca ccaacctggc ttccagcacc
1501 attgtgaagg agggagccaa caaggaggtg ctgggaatcc tagtatccta cagggtcaag
1561 gtgaagctgg tggtgtctcg aggcggggat gtctccgtgg agctaccttt cgtcctaatg
1621 caccccaagc cccacgacca catcaccctt ccccgacccc agtcagcccc ccgggaaata
1681 gacatccctg tggataccaa cctcattgaa ttcgatacca actatgccac agacgacgac
1741 atcgtgtttg aggactttgc gaggcttcgg ctgaagggga tgaaggatga cgactgtgat
1801 gaccagttct gccgggcccg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag
1861 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
1921 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
1981 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
2041 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
2101 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
2161 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
2221 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
2281 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
2341 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
2401 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
2461 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
2521 gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca
2581 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
2641 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
2701 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
2761 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc
2821 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
2881 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
2941 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
3001 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
3061 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
3121 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
3181 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
3241 cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
3301 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
3361 tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt
3421 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
3481 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
3541 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
3601 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
3661 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
3721 gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga
3781 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
3841 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
3901 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
3961 aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
4021 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
4081 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
4141 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
4201 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
4261 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc
4321 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
4381 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
4441 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
4501 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
4561 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
4621 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
4681 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
4741 agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag
4801 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg
4861 ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc
4921 attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg
4981 aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact
5041 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
5101 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
5161 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
5221 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
5281 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
5341 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
5401 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
5461 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
5521 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
5581 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
5641 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
5701 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
5761 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
5821 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
5881 ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
5941 tatatggagt tccg
//